Josh Flitter 
 as Ace Ventura, Jr.

Emma Lockhart 
 as Laura Wilson

Ann Cusack 
 as Melissa Ventura

Cullen Douglas 
 as Dr. Sickinger

Art LaFleur 
 as Russell Hollander

Austin Rogers 
 as A Plus

Reed Alexander 
 as Quenton Pennington, Jr.

Carina Conti 
 as Tara

Ralph Waite 
 as Grandpa Ventura

Ashley Milchman 
 as Emo Kid #1

Brooke Milchman 
 as Emo Kid #2

Aubrey Shea 
 as Daniella

Morgan Curschman 
 as Girl Swimmer #1

Paige Huston 
 as Girl Swimmer #2

Lisa Glaze 
 as Teacher

Kendall Ganey 
 as Little Girl at Spelling Bee

Tom Hillmann 
 as Trooper

Trevin Lica 
 as Bully

Jabari Ty'ree Shaffer 
 as Boy

Jeff Chase 
 as Pennington's Bodyguard

John Connon 
 as Tour Guide

Veryl Jones 
 as Museum Security Guard

Phil Ramirez 
 as Prosecutor

Jim R. Coleman 
 as Judge

Karel Wright 
 as Tabby's Handler

Jeff Farley 
 as Tabby's Security Officer

William Haze 
 as Magician

Terry Loughlin 
 as Horse Owner

Patrick Hoover 
 as Party Kid #1

Haley Sehgal 
 as Party Kid #2

Connor Boyle 
 as Overwhelmed Kid

Brian Patrick Clarke 
 as Quenton Pennington Sr.

Adam Vernier 
 as Agent #1

Tom Brooks 
 as Agent #2

Shane Costa 
 as Pennington's Bodyguard

Cristina Huang 
 as Park Guest

Dakota Marooney 
 as Kid at Party

Brandon Migliore 
 as Crash Car Driver

Rhiannon Roberts 
 as Park Guest

Chris Adler 
 as Pennington's Bodyguard (uncredited)

Karl Anthony 
 as Laura's Dad (uncredited)

Jennifer Badger 
 as Security Team Leader (uncredited)

Sam Bauso 
 as FBI Agent (uncredited)

Jessica Blackmore 
 as Assistant with White Horse (uncredited)

Lisa Buckley 
 as Miss Foster - Ox's Pet Store Clerk (uncredited)

Chase "Uzzle" Carter 
 as FBI Agent (uncredited)

Omar Elkalyoubie 
 as Chinese Dignitary (uncredited)

Bayley Ellenburg 
 as Witness (uncredited)

Emily Rose Everhard 
 as Additional Voices (voice) (uncredited)

Jen Halbert 
 as Tabby's Entourage (uncredited)

Annie Kidwell 
 as County Prisoner (uncredited)

Jesse Kozel 
 as Neighbor (uncredited)

Noah Kronenberger 
 as Park Guest (uncredited)

Jennifer Lonardo 
 as Teacher (uncredited)

Sam Lonigro 
 as Girls Swim Team Member (uncredited)

Leslie Maine 
 as FBI Agent (uncredited)

Jeremy Palko 
 as CSI Agent (uncredited)

Noah Schnacky 
 as Swim Team Boy (uncredited)

David W. Scott 
 as Pennington's Bodyguard (uncredited)

Andy Ussach 
 as Pennington's Butler (uncredited)

Justin Brown 
 as Churro Guy (uncredited)