Tobey Maguire 
 as Spider-Man / Peter Parker

Willem Dafoe 
 as Green Goblin / Norman Osborn

Kirsten Dunst 
 as Mary Jane Watson

James Franco 
 as Harry Osborn

Cliff Robertson 
 as Ben Parker

Rosemary Harris 
 as May Parker

J.K. Simmons 
 as J. Jonah Jameson

Joe Manganiello 
 as Flash Thompson

Gerry Becker 
 as Maximilian Fargas

Bill Nunn 
 as Joseph 'Robbie' Robertson

Jack Betts 
 as Henry Balkan

Stanley Anderson 
 as General Slocum

Ron Perkins 
 as Dr. Mendel Stromm

Michael Papajohn 
 as Carjacker

K.K. Dodds 
 as Simkins

Ted Raimi 
 as Hoffman

Bruce Campbell 
 as Ring Announcer

Elizabeth Banks 
 as Miss Brant

John Paxton 
 as Houseman

Tim DeZarn 
 as Philip Watson

Taylor Gilbert 
 as Madeline Watson

Randy Poffo 
 as Bone Saw McGraw

Larry Joshua 
 as Wrestling Promoter

Timothy Patrick Quill 
 as Wrestling Arena Guard

Lisa Danielle 
 as Bone-ette

Natalie T. Yeo 
 as Bone-ette

Erica D. Porter 
 as Bone-ette

Kristen Davidson 
 as Bone-ette

Jason Padgett 
 as Flash's Crony

Shan Omar Huey 
 as Teacher

Sally Levi 
 as Girl on Bus

Evan Arnold 
 as Doctor

Jill Sayre 
 as Nurse

Jim Ward 
 as Project Coordinator

David Holcomb 
 as Test Pilot

Octavia Spencer 
 as Check-In Girl

Brad Grunberg 
 as Heckler

Shane Habberstad 
 as Little Billy

Deborah Wakeham 
 as Billy's Mom

Rachael Bruce 
 as Times Square Child

Mackenzie Bryce 
 as Times Square Child

Julia Barrie 
 as Times Square Child

Macy Gray 
 as Macy Gray

Myk Watford 
 as Cop at Fire

Bill Calvert 
 as Fireman

Sylva Kelegian 
 as Mother at Fire

Kristen Marie Holly 
 as Young Lady at Fire

Ajay Mehta 
 as Cabbie

Peter Appel 
 as Cabbie

Scott Spiegel 
 as Marine Cop

Matt Smith 
 as Cop at Carjacking

Sara Ramirez 
 as Cop at Carjacking

Lucy Lawless 
 as Punk Rock Girl

Jayce Bartok 
 as Subway Guitarist

Maribel González 
 as Lady Dogwalker

Amy Bouril 
 as Office Lady

Joseph D'Onofrio 
 as Opinionated Cop

Jim Norton 
 as Surly Truck Driver

Corey Mendell Parker 
 as Chaperone in Tram

Ashley Edner 
 as Girl in Tram

William Joseph Firth 
 as Boy in Tram

Alex Black 
 as Boy in Tram

Laura Gray 
 as Tram Group Mother

Joe Virzi 
 as New Yorker on Bridge

Michael Edward Thomas 
 as New Yorker on Bridge

Jeanie Fox 
 as New Yorker on Bridge

Robert Kerman 
 as Tugboat Captain

Stan Lee 
 as Man in Fair (uncredited)

Una Damon 
 as Lab Tour Guide (uncredited)

Rick Avery 
 as Cop (uncredited)

Peter Aylward 
 as Colonel (uncredited)

Jillian Clare 
 as Crying Girl in Tram (uncredited)

Chris Coppola 
 as Kyle (uncredited)

Jesse Heiman 
 as Spectator at School Fight (uncredited)

Leroy Patterson 
 as World Unity Festival Attendant (uncredited)

Benny Urquidez 
 as Mugger (uncredited)

Scott L. Schwartz 
 as Screaming Wrestler (uncredited)

Jophery C. Brown 
 as Chef (uncredited)

Brian J. Williams 
 as Young Thug #2 (uncredited)

Mark De Alessandro 
 as Cop (uncredited)

Tia Dionne Hodge 
 as Uptown Woman (uncredited)

Loren Janes 
 as Board of Directors Member (uncredited)

Andray Johnson 
 as Balkan's Aide (uncredited)

Martin Pfefferkorn 
 as Thug on 8x10 (uncredited)

Tammi Sutton 
 as Street Vendor (uncredited)

Lindsay Thompson 
 as Mary Jane's Friend (uncredited)

Sean Valla 
 as Boat Light Man (uncredited)

Pete Macnamara 
 as Pentagon Officer (uncredited)

Al Goto 
 as Cop (uncredited)

Joni Avery 
 as Cop (uncredited)

Sam Raimi 
 as Popcorn Thrower (uncredited)

Craig 'Radioman' Castaldo 
 as Man with Bike (uncredited)