Tobey Maguire 
 as Spider-Man / Peter Parker

Kirsten Dunst 
 as Mary Jane Watson

James Franco 
 as Harry Osborn

Alfred Molina 
 as Doc Ock / Otto Octavius

Rosemary Harris 
 as May Parker

J.K. Simmons 
 as J. Jonah Jameson

Donna Murphy 
 as Rosalie Octavius

Daniel Gillies 
 as John Jameson

Dylan Baker 
 as Dr. Curt Connors

Bill Nunn 
 as Joseph 'Robbie' Robertson

Vanessa Ferlito 
 as Louise

Aasif Mandvi 
 as Mr. Aziz

Willem Dafoe 
 as Green Goblin / Norman Osborn

Cliff Robertson 
 as Ben Parker

Ted Raimi 
 as Hoffman

Elizabeth Banks 
 as Miss Brant

Bruce Campbell 
 as Snooty Usher

Gregg Edelman 
 as Dr. Davis

Elya Baskin 
 as Mr. Ditkovitch

Mageina Tovah 
 as Ursula

Daniel Dae Kim 
 as Raymond

Hal Sparks 
 as Elevator Passenger

Joel McHale 
 as Mr. Jacks

Stan Lee 
 as Man Dodging Debris

Kelly Connell 
 as Dr. Isaacs

Brent Briscoe 
 as Garbage Man

Emily Deschanel 
 as Receptionist

Jason Fiore-Ortiz 
 as Henry Jackson

Scott Spiegel 
 as Man on Balcony

Andy Bale 
 as OsCorp Executive

Christine Estabrook 
 as Mrs. Jameson

Molly Cheek 
 as Society Woman

John Paxton 
 as Houseman

Joy Bryant 
 as Woman at Web

Joanne Baron 
 as Skeptical Scientist

Peter McRobbie 
 as OsCorp Representative

Timothy Jerome 
 as Injured Scientist

Taylor Gilbert 
 as Mrs. Watson

Peter Vouras 
 as Stage Manager

Donnell Rawlings 
 as Pizza "Heist" Witness

Zachry Rogers 
 as Boy saved by Spider-Man

Ella Rogers 
 as Girl saved by Spider-Man

Louis Lombardi 
 as Poker Player

Marc John Jefferies 
 as Amazed Kid

Roshon Fegan 
 as Amazed Kid

Brendan Patrick Connor 
 as Theater Traffic Cop

Reed Diamond 
 as 'Algernon'

Dan Callahan 
 as 'Jack'

Elyse Dinh 
 as Violinist

John Landis 
 as Doctor

Tim Storms 
 as Chainsaw Doctor

Susie Park 
 as Clawing Nurse

Patricia M. Peters 
 as Screaming Nurse

Michael Edward Thomas 
 as Man at Fire

Anne Betancourt 
 as Woman at Fire

Venus Lam 
 as Child in Burning Building

Bill E. Rogers 
 as Fireman

Joe Virzi 
 as Fireman

Tom Carey 
 as Train Conductor

Jopaul Van Epp 
 as Boy with Mask

Weston Epp 
 as Boy with Mask

Peter Allas 
 as Train Passenger

Brianna Brown 
 as Train Passenger

Bill Calvert 
 as Train Passenger

Tony Campisi 
 as Train Passenger

Joey Diaz 
 as Train Passenger

Chloe Dykstra 
 as Train Passenger

Simone Gordon 
 as Train Passenger

Dan Hicks 
 as Train Passenger

Julia Max 
 as Train Passenger

Savannah Pope 
 as Train Passenger

Timothy Patrick Quill 
 as Train Passenger

Jill Sayre 
 as Train Passenger

Rickey G. Williams 
 as Train Passenger

Michael Arthur 
 as NYPD Officer (uncredited)

Frank Bonsangue 
 as Pizza Man (uncredited)

Cindy Cheung 
 as Chinese Daughter (uncredited)

Phil LaMarr 
 as Train Passenger (uncredited)

Andre M. Johnson 
 as Fireman 2 (uncredited)

Peter Cincotti 
 as Piano Player in Planetarium (uncredited)

Peyton List 
 as Little Girl Playing on Steps (uncredited)

Spencer List 
 as Little Boy Playing on Steps (uncredited)

Troy Metcalf 
 as Blue Collar Guy (uncredited)

Scott Ross 
 as Pedestrian (uncredited)

Bonnie Somerville 
 as Screaming Woman (uncredited)

Wesley Volcy 
 as Columbia University Student (uncredited)

Lou Volpe 
 as Man at Web (uncredited)

Garrett Warren 
 as Bearded Doctor (uncredited)

Joseph M. Caracciolo 
 as Priest at Mary Jane's Wedding (uncredited)

David Boston 
 as Pedestrian (uncredited)

John Cameron 
 as Man Buying 'Spider-Man No More' Newspaper (uncredited)

Sho Brown 
 as Passenger (uncredited)

Sal Ardisi 
 as Parent (uncredited)

Edward Johnson 
 as Planetarium (uncredited)

Grant Curtis 
 as Hot Dog Vendor (uncredited)

Vince Cupone 
 as Victim (uncredited)

William E. Corcoran 
 as Stock Broker (uncredited)

Mohammed Hassan 
 as Middle Eastern Taxi Driver (uncredited)

Rachel Lehrer 
 as Hot Girl (uncredited)

Gene LeBell 
 as Train Passenger (uncredited)

Lorelei Llee 
 as Bank Employee (uncredited)

Joseph Nelson 
 as Extra (uncredited)

Claudia Katz Minnick 
 as Robbie's Assistant (uncredited)

Ronn Ozuk 
 as Cop (uncredited)

Denney Pierce 
 as Train Passenger (uncredited)

Carla Rhodes 
 as Phone Booth Girl (uncredited)

Randy Reinholz 
 as Train Passenger (uncredited)

Rachelle Roderick 
 as Jane, Engineer (uncredited)

Angel Katherine Taormina 
 as Female Onlooker on Street (uncredited)

Douglas Swander 
 as Train Passenger (uncredited)

Jimmy Star 
 as Reporter (uncredited)

Jack Wetherall 
 as Train Passenger (uncredited)

Ken Wharton 
 as Uptight Businessman (uncredited)

Henry Truong 
 as Doc Ock Lab Assistant #2 (uncredited)

Al Collado 
 as Cab Driver (uncredited)

Lee Cogburn 
 as Train Passenger (uncredited)

Sam Raimi 
 as University Student with Bag (uncredited)