Tobey Maguire 
 as Spider-Man / Peter Parker

Kirsten Dunst 
 as Mary Jane Watson

James Franco 
 as New Goblin / Harry Osborn

Thomas Haden Church 
 as Sandman / Flint Marko

Topher Grace 
 as Venom / Eddie Brock

Bryce Dallas Howard 
 as Gwen Stacy

Rosemary Harris 
 as May Parker

J.K. Simmons 
 as J. Jonah Jameson

James Cromwell 
 as Captain Stacey

Dylan Baker 
 as Dr. Curt Connors

Theresa Russell 
 as Emma Marko

Bill Nunn 
 as Joseph 'Robbie' Robertson

Bruce Campbell 
 as Maître D'

Elizabeth Banks 
 as Miss Brant

Ted Raimi 
 as Hoffman

Perla Haney-Jardine 
 as Penny Marko

Willem Dafoe 
 as Green Goblin / Norman Osborn

Cliff Robertson 
 as Ben Parker

Elya Baskin 
 as Mr. Ditkovitch

Mageina Tovah 
 as Ursula

John Paxton 
 as Houseman

Becky Ann Baker 
 as Mrs. Stacy

Stan Lee 
 as Man in Times Square

Michael Papajohn 
 as Dennis Carradine / Carjacker

Joe Manganiello 
 as Flash Thompson

Hal Fishman 
 as Anchorman

Lucy Gordon 
 as Jennifer Dugan

Steve Valentine 
 as Photographer

Tim Maculan 
 as Play Director

Marc Vann 
 as Play Producer

Joe Bays 
 as Jazz Club Manager

Gregg Daniel 
 as Precinct Detective

Rogelio T. Ramos 
 as Emergency Room Doctor

Timothy Patrick Quill 
 as Crane Operator

Menachem Mendel Boymelgreen 
 as Kid in Times Square

Nasir Stewart 
 as Kid in Times Square

Austin Hendrickson 
 as Kid in Times Square

Taylor Hemhauser 
 as Kid in Times Square

Kathryn Bryding 
 as Woman Outside Theater

Joe Virzi 
 as Police Detective

Bill E. Rogers 
 as New Jersey State Policeman

Michael Alexander 
 as New Jersey State Policeman

April Parker Jones 
 as Test Site Technician

Edward Padilla 
 as Test Site Technician

Robert Curtis Brown 
 as Test Site Technician

Paul Terrell Clayton 
 as Test Site Technician

Carolyn Neff 
 as Test Site Technician

Christina Cindrich 
 as Test Site Technician

Sonya Maddox 
 as ICU Nurse

Andre B. Blake 
 as Crane Disaster Radio Policeman

Derrick 'Phoenix' Thomas 
 as Cop at Crane Disaster

Jessi Collins 
 as Mary Jane's Replacement

Michael McLaughlin 
 as Boy at Key to the City Ceremony

Anne Gartlan 
 as Councilwoman

Emilio Rivera 
 as Policeman at Sand Truck

Keith Woulard 
 as Policeman at Sand Truck

Reynaldo Gallegos 
 as Armored Car Driver

Jim Coope 
 as Newstand Patron

Dean Edwards 
 as Newsstand Patron

Margaret Laney 
 as Newsstand Patron

Toni Wynne 
 as Congratulatory Woman at Daily Bugle

Aimee Miles 
 as Coffee Shop Waitress

Tanya Bond 
 as Jazz Club Waitress

Mark Kubr 
 as Jazz Club Bouncer

Emma Raimi 
 as Girl With Camera

Lorne Raimi 
 as Boy at the Final Battle

Henry Raimi 
 as Boy at the Final Battle

Alan Cohn 
 as Jazz Club Musician

Dan Callahan 
 as Jazz Club Musician

Daniel Cummings 
 as Jazz Club Musician

Ron King 
 as Jazz Club Musician

Carol Chaikin 
 as Jazz Club Musician

Vance Hammond 
 as Jazz Club Musician

Frank Anello 
 as NYPD Officer (uncredited)

Anya Avaeva 
 as Model (uncredited)

David Backus 
 as Bucket Boy (uncredited)

Tony Besson 
 as Photoshoot Client (uncredited)

Michael Ciesla 
 as Photographer (uncredited)

Irina Costa 
 as Jazz Club Waitress (uncredited)

John Crann 
 as Theater Patron (uncredited)

Crystal Marie Denha 
 as Jazz Club Girl (uncredited)

Amy V. Dewhurst 
 as Columbia Grad Student (uncredited)

Keith Fausnaught 
 as Pedestrian (uncredited)

Logan Fry 
 as Automobile Driver (uncredited)

Tony Galtieri 
 as News Spectator (uncredited)

Brian Hopson 
 as News Reporter (uncredited)

Andrew James Jones 
 as Jazz Club Bouncer (uncredited)

Natalie Jones 
 as Girl at Key Ceremony (uncredited)

Christopher Jude 
 as Student (uncredited)

Brittany Krall 
 as ER Nurse (uncredited)

Pierangeli Llinas 
 as Woman in Court (uncredited)

Bernadette Lords 
 as British Publicist (uncredited)

Natalie McNeil 
 as Girl Screaming in Camaro (uncredited)

Martha Millan 
 as Oscorp Receptionist (uncredited)

Michele-Nanette Miller 
 as Girl in Times Square (uncredited)

Claudia Katz Minnick 
 as Robbie's Assistant (uncredited)

Jen Oda 
 as Beautiful Girl (uncredited)

Anjelia Pelay 
 as Beautiful Woman (uncredited)

Nick Poltoranin 
 as Police Officer (uncredited)

Vanessa Reseland 
 as Beautiful Woman (uncredited)

La Rivers 
 as Beautiful Woman (uncredited)

Bria Roberts 
 as Businesswoman (uncredited)

Vanessa Ross 
 as Sandman Victim (uncredited)

Brenna Roth 
 as Bad Girl (uncredited)

Shade Rupe 
 as City Hall Cheerer (uncredited)

Daniel Shafer 
 as Jazz Club Guest (uncredited)

Abbey Skinner 
 as Café Girl (uncredited)

Kristin Somo 
 as Jazz Club Patron (uncredited)

Jennifer Sparks 
 as Jazz Club Waitress (uncredited)

Christopher Stadulis 
 as NY Firefighter (uncredited)

Aija Terauda 
 as Model (uncredited)

Evelyn Vaccaro 
 as Beautiful Woman (uncredited)

Nick Vlassopoulos 
 as Bar Patron #5 (uncredited)

Sincerely A. Ward 
 as Model (uncredited)

Jennifer Weston 
 as ER Nurse (uncredited)

Ray Wineteer 
 as Spectator (uncredited)

Emily Eckes 
 as Beautiful Woman (uncredited)

Samantha Ressler 
 as Girl at the Final Battle

Craig 'Radioman' Castaldo 
 as Radioman (uncredited)