Steve Carell 
 as Gru (voice)

Kristen Wiig 
 as Lucy (voice)

Benjamin Bratt 
 as Eduardo/El Macho (voice)

Miranda Cosgrove 
 as Margo (voice)

Russell Brand 
 as Dr. Nefario (voice)

Ken Jeong 
 as Floyd (voice)

Steve Coogan 
 as Silas (voice)

Elsie Fisher 
 as Agnes (voice)

Dana Gaier 
 as Edith (voice)

Moisés Arias 
 as Antonio (voice)

Nasim Pedrad 
 as Jillian (voice)

Kristen Schaal 
 as Shannon (voice)

Pierre Coffin 
 as Kevin / Bob / Stuart / Additional Minions / Evil Minions (voice)

Chris Renaud 
 as Additional Minions / Evil Minions / Italian Waiter (voice)

Nickolai Stoilov 
 as Arctic Lab Guards (voice)

Vanessa Bayer 
 as Flight Attendant (voice)

Ava Acres 
 as Additional Voices (voice)

Lori Alan 
 as Additional Voices (voice)

Jack Angel 
 as Additional Voices (voice)

Eva Bella 
 as Additional Voices (voice)

Georgia Cook 
 as Additional Voices (voice)

John Cygan 
 as Additional Voices (voice)

Debi Derryberry 
 as Additional Voices (voice)

Jess Harnell 
 as Additional Voices (voice)

Danny Mann 
 as Additional Voices (voice)

Mona Marshall 
 as Additional Voices (voice)

Mickie McGowan 
 as Additional Voices (voice)

Mason McNulty 
 as Additional Voices (voice)

Alec Medlock 
 as Additional Voices (voice)

Laraine Newman 
 as Additional Voices (voice)

Jan Rabson 
 as Additional Voices (voice)

Andre Robinson 
 as Additional Voices (voice)

Katie Silverman 
 as Additional Voices (voice)

Casey Simpson 
 as Additional Voices (voice)

Claira Nicole Titman 
 as Additional Voices (voice)

Jim Ward 
 as Additional Voices (voice)

April Winchell 
 as Additional Voices (voice)

Joe Matthews 
 as Additional Voices (voice)